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Red cell genotyping


The IBGRL Molecular Diagnostics department offers United Kingdom Accreditation Service (UKAS) accredited tests to national and international customers.

In multi-transfused patients or DAT positive patients, the presence of transfused red cells or auto-antibodies can prevent determination of blood group phenotype by serological techniques, however genetic analysis can be used to predict blood group phenotype.  

Red cell genotyping from blood, amniotic fluid or chorionic villi

IBGRL Molecular Diagnostics uses various techniques to determine blood group genotype: Allele-specific PCR, real-time PCR (allelic discrimination using Taqman probes) and HEA Beadchip. The technique used will depend on the test requested and the current workload in the laboratory.

Available genotyping tests

Standard Blood Group Genotype 

RhD, c, C, E, e, K/k, Jka /Jkb, Fya /Fyb, MN, S/s, U-/ Uvar

Extended Blood Group Genotype

RhD, c, C, E, e, V, VS, K/k, Jka /Jkb, Fya /Fyb, Fyx, MN, S/s, U-/ Uvar, Doa /Dob, Jsa /Jsb, Kpa /Kpb, Lua /Lub, Coa /Cob, Dia /Dib, Sc1/ Sc2, LWa /LWb

Extended Genotype: Haemoglobinopathy Patient Array 

This genotyping test array is particularly suited for haemoglobinopathy patients.

RhD, C, c, E, e, (including common RhD, C and e variants), V, VS, hrB, hrS, K/k, Kpa /Kpb, Jsa /Jsb, Doa /Dob, Fya /Fyb, Jka /Jkb, M/N, S/s, U-, Uvar

Blood Group Genotyping (patients and donors)

Please note that standard patient genotyping requests can be performed urgently, (<10 working days and within 48 hours), for an added premium of £125.00

Investigation
Investigation
Notes
Notes
Samples required (minimum 0.5ml EDTA blood)
Samples required (minimum 0.5ml EDTA blood)
Accompanying form
Accompanying form
Turnaround times
Turnaround times
Standard Genotyping
Notes
(RhD, C, c, E, e, K/k, Fya/b, Jka/b, M/N, S/s, U-, Uvar)
Samples required (minimum 0.5ml EDTA blood)
3ml EDTA blood

or 5ml Amniotic fluid

For CV send pre-extracted DNA (min conc of 10ng/µl & min volume 60µl)

Accompanying form
FRM4738

Guidance

Turnaround times
Within 10 business days (or 48 hours for an added premium)
Extended Genotyping
Notes
RhD, C, c, E, e, V, VS, K/k, Kpa/b,Jsa/b, Fya/b, FyX, Jka/b, M/N, S/s, U-, Uvar , Lua/b, Dia/b, Coa/b, Doa/b, LWa/b, Sc as required
Samples required (minimum 0.5ml EDTA blood)
3ml EDTA blood

or 5ml Amniotic fluid

For CV send pre-extracted DNA (min conc of 10ng/µl & min volume 60µl)

Accompanying form
FRM4738

Guidance

Turnaround times
Within 10 business days
Extended Genotyping (haemoglobinopathy array)
Notes
RhD, C, c, E, e, (including common RhD, C and e variants),V, VS, hrB, hrS , K/k, Kpa/b, Jsa/b,Doa/b, Fya/b, Jka/b, M/N, S/s, U-, Uvar
Samples required (minimum 0.5ml EDTA blood)
3ml EDTA blood
Accompanying form
FRM1597
Turnaround times
Within 12 weeks

Additional Information

User guide

Download our user guide for referring samples to IBGRL Molecular Diagnostics.

Download guide (PDF)

Have a question?

Please contact the laboratory or our business development manager for more information:

If you wish to set up a contract to send samples or would like to obtain information regarding the tests performed, please contact Erika Rutherford.

For all other enquiries, please contact Molecular Diagnostics.