Fetal genotyping – diagnostic
Non-invasive fetal genotyping for mothers with red cell antibodies and non-invasive fetal sex typing
The IBGRL Molecular Diagnostics department offers United Kingdom Accreditation Service (UKAS) accredited fetal blood group genotyping tests to national and international customers.
The laboratory offers a non-invasive, convenient and reliable service for women who have red cell allo-antibodies and may be affected by haemolytic disease of the fetus and newborn (HDFN). Using cell free fetal DNA (cffDNA) in maternal blood, the fetal RhD, RhC, Rhc, RhE and K status can be predicted.
The laboratory also conducts a fetal sex genotyping service for pregnancies affected by X-linked genetic conditions or when early treatment of the fetus differs according to fetal gender.
The fetal genotyping test is available for customers (who do not have a Blood and Blood Component contract with NHSBT) under our Terms and Conditions contract by signing our referral form. Please contact our Business Development Manager Erika Rutherford firstname.lastname@example.org for more information.
|Investigation||Notes||Sample(s) required (minimum 0.5ml EDTA blood)||Accompanying Form||Turnaround times|
|Fetal Genotyping from Maternal Blood||Pregnancy must be at least 16 weeks for RhD/C/E/c Pregnancy must be at least 20 weeks for Kell typing.||16ml EDTA blood per Rh test plus 3ml paternal blood if available (for RhD only)||FRM4674||Within 7 business days|
|Fetal Sex Typing from Maternal Blood||Pregnancy must be at least 7 weeks.||16ml EDTA blood||FRM4739||Within 5 business days|
Sending frozen plasma
Samples can also be sent as aliquots of frozen plasma prepared to a protocol provided by IBGRL – see INF1291. Please contact the laboratory prior to sending frozen plasma. Frozen plasma aliquots that do not meet the sample labelling requirements listed in INF1291 will not be tested.
Fetal Genotyping from Amniotic Fluid or Chorionic Villus
|Investigation||Notes||Sample(s) required||Accompanying Form||Turnaround times|
|Fetal Genotyping from Amniotic Fluid or Chorionic Villus||N/A||
5ml Amniotic fluid
For CV send pre-extracted DNA (min conc of 10ng/µl & min volume 60µl)
|FRM4738||Within 10 business days|
To avoid the possibility of contamination, it is preferable to dispatch the amniotic fluid without transferring it to a second container. If amniotic fluid is transferred from one container to another, then precautions should be taken to avoid contamination with material containing exogenous DNA.
Pre-extracted DNA must be referred. The laboratory performing the DNA extraction should ensure that procedures are in place to prevent contamination of the DNA samples with extraneous DNA or other substances. DNA must be at a minimum concentration of 10ng/µl and a minimum volume of 60µl.
Download our user guide for referring samples to IBGRL Molecular Diagnostics
Have a question?
Please contact the laboratory or our business development manager for more information:
If you wish to set up a contract to send samples or would like to obtain information regarding the tests performed, please contact Erika Rutherford.
For all other enquiries, please contact Molecular Diagnostics.