IBGRL Molecular Diagnostics

      

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Red Cell Reference

The International Rare Donor Panel 

Molecular Diagnostics User Guides Sending Samples Molecular Diagnostics

IMPORTANT: Hospitals and reference centres wishing to refer for the first time must contact the Molecular Diagnostics laboratory before sending the sample to discuss the appropriateness of the proposed investigation. 
Samples referred without the consent of the Molecular Diagnostics laboratory may not be investigated.

Contact Details   Address Details
Kirstin Finning (Head of Molecular Diagnostics) Tel: +44 (0)117 921 7573
Sarah Tarrant (Laboratory Manager) Tel: +44 (0)117 921 7572 
Laboratory Tel: +44 (0)117 921 7602
Email: molecular.diagnostics@nhsbt.nhs.uk
FAX : +44 (0) 117 912 5782

Working Hours
Our normal working hours are:
Monday - Friday 9.00am - 5.00pm

Molecular Diagnostics IBGRL,
NHS Blood and Transplant
North Bristol Park
Filton
Bristol
BS34 7QH
UK

 

Investigation Sample(s) required

Accompanying Form

 

Fetal Genotyping from Maternal Blood

Pregnancy must be at least 16 weeks for RhD/C/E/c

Pregnancy must be at least 20 weeks for Kell typing.

16ml EDTA blood per Rh test
(plus 3ml EDTA blood from partner, if available)

16ml EDTA blood

 

FRM4674

Guidance for completion:
INF1343

 
Fetal Genotyping from Amniotic Fluid or Chorionic Villus

5ml Amniotic fluid

For CV (contact the Genotyping laboratory)   Send a DNA sample (min conc 10ng/l & min volume 60l)


FRM4738

Guidance for completion:
INF1341

 

Fetal Sex Typing from Maternal Blood

Pregnancy must be at least 7 weeks.




16ml EDTA blood

FRM4739

Guidance for completion:
INF1342
 
Blood Group Genotyping (patients and donors)    

Please note that standard patient genotyping requests can be performed urgently, (< 10 working days and within 24hours), for an added premium of 125.00

Standard Genotyping
(RhD, C, c, E, e, K/k, Fya/b, Jka/b, M/N, S/s, U-, Uvar)
3ml EDTA blood
(minimum 0.5ml EDTA blood)
FRM4738

Guidance for completion:
INF1341
 
Extended Genotyping
RhD, C, c, E, e, V, VS, K/k, Kpa/b,Jsa/b, Fya/b, FyX, Jka/b, M/N, S/s, U-, Uvar , Lua/b, Dia/b, Coa/b, Doa/b, LWa/b, Sc as required
3ml EDTA blood
(minimum 0.5ml EDTA blood)
FRM4738

Guidance for completion:
INF1341
 
Extended Genotyping
(haemoglobinopathy array):
RhD, C, c, E, e, (including common RhD, C and e variants),V, VS, hrB, hrS , K/k, Kpa/b, Jsa/b,Doa/b, Fya/b, Jka/b, M/N, S/s, U-, Uvar
3ml EDTA blood
(minimum 0.5ml EDTA blood)
FRM1597  

 

Referring UK Laboratories

Referring International Laboratories

Samples should be sent by first class mail or courier to ensure arrival, between Monday and Friday, within 48hours of the sample being taken. Blood samples can be sent at room temperature.

Samples which have lysed due to prolonged time in transit, or which are not in the desired anti-coagulant (See table above), will not be tested.

Please complete and return the appropriate form (See table above).

Please indicate the urgency of the investigation and the infection risk status, if known.

Information regarding the ethnic origin of the patient and partner and previous relevant clinical history is very useful for interpretation of results.

Please indicate which clinician the result should be reported (other than the referring laboratory) and where the invoice should be addressed.

Samples MUST be labelled, as a minimum, with the three forms of identification - Name, Date of Birth, Hospital/NHS Number.

Please ensure that the information on samples matches the information on accompanying forms (See table above).

Reporting Results to UK Laboratories

Results are generally available within 5 working days of receipt, quicker turn around times are possible for urgent samples - please enquire (See details above).

Samples submitted through the NHSBT Laboratory Information Management System (Hematos) will be reported through Hematos.

Reports available to view on the Sp-ICE system in PDF format:

  • Fetal genotyping from maternal plasma
  • Full genotyping for common blood group antigens
  • Sex typing

If you do not currently have access to the Sp-ICE browser and wish to view these reports, please contact your local Transfusion Laboratory Manager (Sp-ICE primary contact) or e-mail ICE@nhsbt.nhs.uk for training and access.

A paper copy of fetal genotyping reports are sent by first class mail. Urgent cases may be reported by fax until October 2015, or by E-mail.

 
 

Samples should be sent by express mail, courier or by air freight ensure arrival, between Monday and Friday, within 48hours of the sample being taken.

If this time frame cannot be met, samples can be sent as aliquots of frozen plasma, but care must be taken to avoid contamination with extraneous DNA. Please contact the Genotyping Laboratory for extraction method details (See details below).

Samples which have lysed due to prolonged time in transit, or which are not in the desired anti-coagulant (See table above), will not be tested.

Please complete and return the appropriate form (See table above).

Please indicate the urgency of the investigation and the infection risk status, if known.

Information regarding the ethnic origin of the patient and partner and previous relevant clinical history is very useful for interpretation of results.

Please indicate which clinician the result should be reported (other than the referring laboratory) and where the invoice should be addressed.

Samples MUST be labelled, as a minimum, with the three forms of identification - Name; Date of Birth; Hospital Number.

Please ensure that the information on samples matches information on accompanying forms (See table above).

Reporting Results to International Laboratories

A paper copy of results will be sent to the clinician indicated on the accompanying form. Urgent cases can be reported by E-mail.